OrangerV1, Main, Exploration, bibRecord, 001602

Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck]

Identifieur interne : 001602 ( Main/Exploration ); précédent : 001601; suivant : 001603

Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck]

Auteurs : XINLEI DA [République populaire de Chine] ; KEQIN YU [République populaire de Chine] ; SHIHUI SHEN [République populaire de Chine] ; YAJIAN ZHANG [République populaire de Chine] ; JUXUN WU [République populaire de Chine] ; HUALIN YI [République populaire de Chine]

Source :

Plant physiology and biochemistry : (Paris) [ 0981-9428 ] ; 2012.

RBID : Pascal:12-0266105

Descripteurs français

Pascal (Inist)
- Identification, Différenciation, Expression génique, Spontané, Forme, Feuille végétal, Mutation, Bourgeon, Hybridation suppressive soustractive, Citrus sinensis, Marqueur EST, Physiologie végétale.

English descriptors

KwdEn :
- Bud, Citrus sinensis, Differentiation, Expressed sequence tag, Gene expression, Identification, Mutation, Plant leaf, Plant physiology, Shape, Spontaneous, Suppression subtractive hybridization.

Abstract

Most of the economically important citrus cultivars have originated from bud mutations. Leaf shape and structure are important factors that impact plant photosynthesis. We found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [Citrus sinensis (L.) Osbeck]. To identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (SSH) and macroarray analysis. A total of 221 non-redundant differentially expressed transcripts were obtained. These transcripts included cell wall- and microtubule-related genes and two transcription factor-encoding genes, yabby and wox, which are crucial for leaf morphogenesis. Many highly redundant transcripts were associated with stress responses, while others, encoding caffeic acid 3-O-methyltransferase (EC 2.1.1.68) and a myb-like transcription factor, might be involved in the lignin pathway, which produces a component of secondary walls. Furthermore, real-time quantitative RT-PCR was performed for selected genes to validate the quality of the expressed sequence tags (ESTs) from the SSH libraries. This study represents an attempt to investigate the molecular mechanism associated with a leaf shape mutation, and its results provide new clues for understanding leaf shape mutations in citrus.

Affiliations:

République populaire de Chine

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Le document en format XML

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<term>Identification</term>
<term>Mutation</term>
<term>Plant leaf</term>
<term>Plant physiology</term>
<term>Shape</term>
<term>Spontaneous</term>
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<term>Expression génique</term>
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<term>Feuille végétal</term>
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<front><div type="abstract" xml:lang="en">Most of the economically important citrus cultivars have originated from bud mutations. Leaf shape and structure are important factors that impact plant photosynthesis. We found a spontaneous bud mutant exhibiting a narrow leaf phenotype in navel orange [Citrus sinensis (L.) Osbeck]. To identify and characterize the genes involved in the formation of this trait, we performed suppression subtractive hybridization (SSH) and macroarray analysis. A total of 221 non-redundant differentially expressed transcripts were obtained. These transcripts included cell wall- and microtubule-related genes and two transcription factor-encoding genes, yabby and wox, which are crucial for leaf morphogenesis. Many highly redundant transcripts were associated with stress responses, while others, encoding caffeic acid 3-O-methyltransferase (EC 2.1.1.68) and a myb-like transcription factor, might be involved in the lignin pathway, which produces a component of secondary walls. Furthermore, real-time quantitative RT-PCR was performed for selected genes to validate the quality of the expressed sequence tags (ESTs) from the SSH libraries. This study represents an attempt to investigate the molecular mechanism associated with a leaf shape mutation, and its results provide new clues for understanding leaf shape mutations in citrus.</div>
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<name sortKey="Shihui Shen" sort="Shihui Shen" uniqKey="Shihui Shen" last="Shihui Shen">SHIHUI SHEN</name>
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Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck]

Identification of differentially expressed genes in a spontaneous altered leaf shape mutant of the navel orange [Citrus sinensis (L.) Osbeck]

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri